A study on complete Blood cell count in young healthy Medical individuals Essay

A survey on complete Blood cell count in immature healthy Medical persons

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

We Will Write a Custom Essay Specifically
For You For Only $13.90/page!


order now

Abstraction

Background:complete blood cell count is a often used research lab trial for the diagnosing of several diseases, in wellness scrutiny and preoperative rating. The values of haematological parametric quantities are affected by a figure of factors even in seemingly healthy population. Recently it was reported that white blood cell count and thrombocyte counts are associated with cardiovascular diseases. It is of involvement to observe that the premenopausal adult females have a lower incidence of cardiovascular disease than work forces perchance due to female sex hormonal consequence on thrombocyte maps. so this survey has been conducted in order to find any sex fluctuation consequence on haematological parametric quantities in seemingly healthy male and female immature grownup topics.

Purposes and aims:The Aim of this survey is to mensurate the assorted haematological values in the immature grownup male and female topics and so to compare their consequences.

Methods:80 immature grownup pupils between 17 to 20 old ages of age group have participated in this survey voluntarily. After being informed blood samples of topics had been taken in forenoon. Hematologic parametric quantities were analysed by Sysmex KX -21 automated haematology analyser. The hematologic alterations in between male and female topics were analyzed by Student’s paired “t” trial severally.

Consequence: It shows that difference between the degrees of Differential leucocyte cell count, Hemoglobin and Platelet count of the two studied group is statistically important ( p & lt ; 0.05 ) . although some blood cell of male and female topics in the same age group were in the mention scope it is thought that their being high or low in figure is related to variant status followed for long continuance of clip.

Cardinal words:Male, Female, Blood cell, Hemoglobin, Leucocyte, Platelet

Writer for correspondence:xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

Introduction

Study of haematological parametric quantities like complete blood cell count is a often used research lab trial performed to back up the diagnosing of several diseases. It is besides used in periodic wellness scrutiny and preoperative rating. The values of haematological parametric quantities are affected by a figure of factors even in seemingly healthy population. These include age, sex, organic structure physiques, and nutritionary, environmental and societal factors with cultural backgrounds.1It has been shown in several surveies that some of the haematological parametric quantities exhibit considerable fluctuation in different period of life. At birth the entire Hb degree, RBC count, PCV are shown to be higher than at any other period of life.2,3The degrees of these parametric quantities so diminish during the following few months after birth, some more steeply than others, with cells going hypo chromic with the development of physiological Fe lack anaemia.4The Hb content and RBC count so bit by bit lift and attacks near to the grownup degrees by the age of pubescence.5In general the male haematological degrees are higher than the grownup female degrees.6However, Tell et Al ( 1985 )7reported that entire WBC & A ; Platelet counts are significantly higher in adolescent female than adolescent male topics of 14-16 old ages of age. Recently it was besides reported that white blood cell count and thrombocyte counts are associated with cardiovascular diseases. Entire WBC and certain subtype counts in immature maturity are significantly associated with the presence of coronary arteria calcification ( CAC ) 15 or 20 old ages subsequently in early in-between age. This suggests possible engagement of WBC in induction or early development of coronary artery disease at ulterior age of life.8Therefore, survey on WBC at an early maturity is of import.

Trial

Male

Female

P-value

Red blood cell

4.76±0.38

4.59±0.29

0.075

Hb ( g/dl )

13.58±1.05

12.67±1.10

0.005*

HCT ( % )

41.96±4.51

39.92±2.70

0.064

MCH ( pg )

28.67±1.90

27.60±1.61

0.041*

MCHC ( g/dl )

32.79±2.24

31.70±0.93

0.033

RDW ( Florida )

46.31±4.55

44.04±2.67

0.036*

Materials and methods

40 healthy females mean 20.75 ± 2.23 old ages old and 40 healthy males, on mean 20.83 ± 0.96 old ages old ; a sum of 80 people seemingly healthy have participated in this survey on a voluntary footing. The topics was excluded from the survey, if they Suffer from any haematological, endocrinological, gynaecological, cardiovascular, respiratory and nervous upsets and grounds of infection at the clip of trying or Capable had history of Blood transfusion or contribution in last 3 months. The protocol has been explained to the topics. 2 milliliter of venous blood sample was drawn between 9 a.m to 12 midday from anticubital vena under sterile safeguards in to a vial containing of 10 % K EDTA to avoid diurnal fluctuations. The sample was analysed instantly within 1-2 hour, to avoid any fluctuations due to storage. Hematologic parametric quantities such as ruddy blood cell count ( CBC ) , hemoglobin ( HBG ) , hematocrit value ( HCT ) , average corpuscular volume ( MCV ) , average cell haemoglobin ( MCH ) , average cell haemoglobin concentration ( MCHC ) , erythrocyte distribution breadth ( RDW ) , leukocyte count ( TLC ) , Differencial cell count, thrombocyte count ( PLT ) were analyzed with “Sysmex-kx-21” trade name blood cell counter device in cardinal research lab of Dhiraj infirmary Piparia from July 2012 to Oct 2012. SPSS plan has been used in appraisal of informations. Consequences have been decided on footing of Mean and Standard Deviation. t trial was applied in independent groups to compare them. 0.05 values were accepted as important.

Consequences

Table 1: Erythrocyte Parameters of Male ( n=40 ) and Females ( n=40 )

Table 2: Leukocyte & A ; Platelet count of Male ( n=40 ) and Females ( n=40 )

Trial

Male

Female

P-value

Entire WBC count

7.48± 1.85

7.02 ± 2.00

0.418

Differential Neutrophil count

65.33 ± 7.83

56.70 ± 13.59

0.010*

Differential Lymphocyte count

29.90 ±9.72

35.80 ± 9.74

0.041*

Differential Monocyte count

5.85 ± 2.16

7.49 ± 1.10

0.002*

Entire Platelet count

218.79 ± 29.96

252.54 ± 37.84

0.001*

Discussion

In our survey Erythrocyte parametric quantities like Hemoglobin, MCH and MCHC have been found to be higher in males, entire WBC count with neutrophil count besides higher in male topics. Whenever lymphocyte count, monocyte count and Platelet were higher in female topic. EL- Hazmi and Warsy ( 2001 ) studied Saudi Children with ages runing from 1-15 old ages. The RBC Count did non Show a Significant Changes in the 1 to 13 twelvemonth Old but rope somewhat beyond this age. No Significant differences were observed in ruddy cell count in the male and female kids. White blood cell bit by bit decreased 2 old ages onwards, While Hemoglobin and haematocrit degrees increased significantly from 2 to 15 old ages. The same writer reported WBC Count 10.9 ± 3.8 tens 1012/L, 6.9 ± 3.1 ten 109/L in one twelvemonth olds, 9-11 old ages & A ; 12-15 old ages age group severally.9Ghafouri et Al ( 1987 ) reported that entire Level of haemoglobin was 13.7 ± 1.0 g/dl And 13.5 ± 1.0 g/dl, severally in male & A ; female kids, with ages runing from 12-15 old ages. Hemoglobin Level was Lowest in the biennial olds, and so bit by bit increased up to 15 old ages of age in both male childs & A ; misss. The differences of Boys & A ; Girls Level was important after 14 old ages of age, The male values were Higher than the female values.10Usman k et Al ( 2007 ) studied 302 healthy voluntaries, both male and female, ages Range between 20-45 old ages. They found, in males, the average Hb concentration of 13.04 g/dl. Was significantly higher than females value of 11.63 g/dl. The RBC Count of 5.3 x1012/L in males was significantly higher than the corresponding Valuess of 4 x1012/L in females. On the other manus The mean WBC Count of 8.25 tens 109/L in males was lower than the average values of 8.42 x109/L in females. Similarly the Valuess for thrombocyte count of 255 x109/L in males were besides Significantly lower than matching values of 279 tens 109/L in females.11Khanduri et Al ( 2005 ) reported thrombocyte counts in 25 males & A ; 25 females normal grownup Indians the scope being 111-338 ( ten 109/L ) and 137-337 ( ten 109/L ) severally.12Earlier Bain ( 1985 ) reported the average thrombocyte counts which were 288 and 262 tens 109/L in Caucasic females and males severally.13Casimir et Al ( 2010 ) reported that Gender influences clinical presentation and markers in inflammatory diseases, in many chronic condititions frequence of complications is greater in females with the addition production of inflammatory markers like CRP ( C – reactive protein ) . Neutrophil count and ESR.14Although legion surveies have been undertaken to analyze the effects of Gender and assorted factor on differential blood counts but consequences have frequently been inconclusive and contradictory ( Makinoda et al,1996 )15

Decision

Although blood cells of both groups are within the mention scope, their being low or high in Numberss is based on assorted factor like age, sex, organic structure physique, and nutritionary, environmental and societal factors with cultural backgrounds. For clearance similar type of surveies with multi topic and multi repeat are needed.

Mentions

  1. Mohson AF, Hazmi EL, Warsy As. Normal mention values for haematological parametric quantities, ruddy cell indices, HbA2 and HbF from early childhood through adolescence in Saudis. AS Thousand 2001 ; 21: 165-169.
  2. Matoth Y, Zaizon R, Varsano I. Postnatal alterations in some ruddy cell parametric quantities. Acta Paediatr Scand 1971 ; 60: 371.
  3. Marwaha N, Marwaha RK, Narang A, Thusu K, Garewal G, Bhakod ON, Routine hematological values in term neonates. Indian Pediatrics 1992 ; 29: 1095-1099.
  4. Dacie JV, Lewis SM. Practical Haematology. 7th edition. Londan: Churchill Livingstone,1991.
  5. Taylor MR, Holland CV, Spencer R, Jackson JF, conner GI, Donnell JR. Haematological mention scope for school kids. Clin Lab Haematol 1997 ; 19: 1-15.
  6. Kelly A, Munan L. Haematological profile of normal populations: ruddy cell parametric quantities. Br J Haemtol 1977 ; 35: 153.
  7. Tell GS, Grimm RH, Vellar OD. The relationship of white cell count, thrombocyte count, and haematocrit to cigarette smoke in striplings: the Oslo Youth Study. Circulation 1985 ; 72: 971-974.
  8. Hou L, Jones DL, Ning H, Huffman MD, Fornage M, He K et Al. White blood cell count in immature maturity and coronary arteria calcification in early in-between age: coronary arteria hazard development in immature grownups ( CARDIA ) survey. Circulation 2012 ; 125: 11.
  9. Gilles HM, Haematology in tropical Africa. Clin Hematol 1981 ; 10: 695-705.
  10. Ghafouri HM, Alfares AM, Islam SI, Ahmed AO, Jan MY. Haematological mention values assessed from birth to adolescent in Saudi topics in the country of Jeddah. Saudi Med J 1987 ; 8: 575-82.
  11. Usman K, Syed ZA, Rao AA. References scope values of haematological parametric quantities in healthy Pakistani grownups. Pak J Physiol 2007 ; 19-22.
  12. Khanduri U, Sharma A and Joshi A. supernatural vitamin B12 and folate lack in Indians. The national medical diary of India, 2005 ; 18 ( 4 ) : 182-183.
  13. Bain BJ Platelet count and thrombocyte size in males and females. Scand J Haematol 1985 ; 35: 77-9.
  14. Casimir G, Muller S, Hanssens L, Zilberg K, Duchateau J. Gender differences in inflammatory markers in babyhood. Shock, 2010, 33 ( 3 ) : 258-262.
  15. Makinoda S, Mikuni M, Sogame M, Kobamastsu Y, Yamada H, Yamamoto R, Fujimoto S, FUruta I. Erythropoietin, granulocyte –colony exciting factor, interlukin-1 beta and interleukin-6 during the catamenial rhythm. Int J Gynecol Obstet 1996 ; 55: 265-71.